Figure 4.

Ackr2 deficiency leads to an increased Th17 response during the induction of arthritis. At 8 days after challenge with CII, draining LNs were harvested from WT littermate control (WT) and Ackr2-deficient (Ackr2^−/−) DBA/1j mice (n=5) and the recall immune response evaluated. Single-cell suspensions of LN cells (3 × 10⁵ cells per well) were stimulated with CII (60 μg ml⁻¹) or medium alone and assessed for (a) specific proliferation (that is, proliferation after CII stimulation minus proliferation in medium alone) or (b, c) the levels of IFNγ and IL-17 in the medium. **P<0.01 by Kruskal–Wallis test with Dunn's multiple comparison post test. A repeat experiment yielded similar results.