Figure 5.

Ackr2 deficiency does not impair disease severity in the MOG_35–55 peptide-induced model of EAE or result in increased IL-17 production. EAE was induced in WT littermate control (WT) and Ackr2-deficient C57BL/6 mice (Ackr2^−/−) by immunization with MOG_35–55 (100 μg) in complete Freund's adjuvant (4 mg ml⁻¹ Mycobacterium tuberculosis H37RA) and treatment on days 0 and 2 with 200 ng of pertussis toxin (PTX). (a) All animals were assessed daily for the development of clinical symptoms of disease. Each point represents the mean±s.e.m. scores for n=5 mice. A repeat experiment yielded similar results. (b) Injection site skin was harvested on day 3 and the number of CD207⁺ DCs in 10 high-power fields (HPFs) per mouse determined. (c–e) Draining LNs were harvested on day 3 or day 11 and single-cell suspensions were evaluated for (c) the absolute number of CD207⁺EPCAM⁺ DCs (day 3) or (d, e) 3 × 10⁵ cells per well stimulated with MOG_35–55 (30 μg ml⁻¹) (day 11) and assessed for (d) specific proliferation (proliferation after MOG_35–55 stimulation minus proliferation in medium alone) or (e) the level of IL-17 in the medium. Significance was determined by t-test. *P<0.05.