A-B) Assessment of recovery of BRIC6 (band 3, A) and LA1818 (RhAG, B) epitopes on cells pretreated with 500μg/ml pronase for 0, 60, 120 and 180 min, as measured by flow cytometry (mean fluorescent intensity (arbitrary units) in differentiation medium at 37°C at indicated periods of time during differentiation (hours). Means and standard deviations represent three experiments. C) Cells were pretreated with 100ng/ml cycloheximide (white symbols) or not (black symbols) prior to pronase treatment. Recovery of BRIC6 (band 3) and LA1818 (RhAG) epitopes was monitored at 0, 60, 120 and 180 min after pronase treatment at specific time points during differentiation. Data expressed as mean fluorescence intensity (arbitrary units) as a function of recovery time from two independent experiments.