Figure 7.

Determination of Ldoc1/Mart7 sense and antisense transcripts using 5′ and 3′ RACE. A) 5′ and 3′ RACE for sense transcripts were performed with a gene specific (GS) sense (s) primer for first strand cDNA synthesis (cDNA) and subsequent PCR. PCR products were analyzed on agarose gels with ethidium bromide, which identified all extended transcripts (left) and are shown in the gene map (right). B) 5′ and 3′ RACE for antisense transcripts were performed with a gene specific (GS) antisense (as) primer for first strand cDNA synthesis (cDNA) and subsequent PCR. PCR products showed all extended transcripts (left) and in the gene map (right).