Fig 5. Fluorescence in situ hybridization coupled with confocal laser scanning microscopy images.

A) Gammaproteobacteria (yellow) and Betaproteobacteria (pink) form large colonies on the lateral roots of young lettuce plantlets, where they do not share their space, moreover it seems they exclude each other. B) We revealed increased gammaproteobacterial (yellow) colonization close to stomata which could be also detected on R. solani infected lettuce. C) Punctiform extensive colonization behavior of Escherichia coli K12 (yellow) on inoculated lettuce patches within the inner compartments of the leafy green. D) Physical damage to the leaves affects the epicuticular wax-layer (black arrow) and the cuticle cells (white arrow), and supports endophytic colonization of enterobacteria (yellow). E) Transport of bacteria (red) within a vascular bundle (white marker) and F) trough stoma.