Skip to main content
. Author manuscript; available in PMC: 2015 Feb 25.
Published in final edited form as: J Gene Med. 2010 Mar;12(3):255–265. doi: 10.1002/jgm.1440

Figure 4.

Figure 4

Specific knockdown of mCherry-CCR5 chimera expression by sh1005 in 293T cells. 293T cells (1 × 105) were cotransfected with lentiviral vectors expressing sh1005 or the muCCR5 shRNA and either mCherry-CCR5 chimera or mCherry encoding lentiviral vector by FuGENE. The cells were then cultured for 2 days and the mCherry expression was analysed by fluorescence microscopy (A) and flow cytometry (B). The results are exhibited as side scatter versus mCherry dot plots. The quadrant lines were defined by mock 293T cells (data not shown) and the percentage numbers are indicated