. Author manuscript; available in PMC: 2015 Feb 25.

Published in final edited form as: J Gene Med. 2010 Mar;12(3):255–265. doi: 10.1002/jgm.1440

Table 1. shRNA transduction of FL-CD34⁺ cells does not grossly affect the colony formation in the methylcellulose assay.

	Total	GM	GEMM	Erythroid	EGFP +	%EGFP
Mock	104	66	36	2	0	0
Luc shRNA	104	53	48	3	29	28
sh1005	83	46	35	2	35	42

FL-CD34+ cells (2 × 10⁵) were transduced with lentiviral vectors expressing sh1005 or Luc shRNA. Viable cells were quantified, plated (2 × 10³ cells/ml), and cultured in humidified chambers for hematopoietic colony-formation using standard methylcellulose medium containing 10 ng/ml of IL-3, 50 ng/ml of SCF, 10 ng/ml of GM-CSF and 3 units/ml of erythropoietin. Cultures were scored at day 14 for granulocyte-macrophage colony (GM), granulocyte, erythroid, macrophage and megakaryocyte (GEMM), and erythroid colony (Erythroid) under the microscope.

Table 1. shRNA transduction of FL-CD34+ cells does not grossly affect the colony formation in the methylcellulose assay.

Table 1. shRNA transduction of FL-CD34⁺ cells does not grossly affect the colony formation in the methylcellulose assay.