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. 2015 Feb 13;15:50. doi: 10.1186/s12870-015-0441-5

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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2-BP abolished the tonoplast localization of CBL2 in root hairs. A-D. 4 DAG seedlings of Pro _UBQ10:CBL2-RFP transgenic plants treated with either DMSO (A, B) or 2-BP (C, D) for 4–12 hr before visualization. E-F. 4 DAG seedlings of Pro _UBQ10:CBL2-RFP;pat10-2 transgenic plants treated with DMSO for 4–12 hr before imaging. Representative initiating (A, C, E) or elongating (B, D, F) root hairs are shown. V indicates vacuole. G. Quantification of CBL2-RFP distribution in the tonoplast v.s. the cytoplasm (Tonoplast/Cyt) at different time points after 2-BP treatment. a.u. stands for arbitrary fluorescence units. Bars = 7.5 μm.