Fig. 2. Kinetic Analysis with of Cyclipostin P Trans (4a).
A: Timecourse for HSL activity in the presence of 10 nM 4a, 80 nM HSL. Data were fit to Eqn 1 to demonstrate that k−2 is essentially zero. See text for details. B: Relative residual HSL activity as a function of cyclipostin P trans (4a) concentration. Data were fit to Eqn 2 to yield KI of 40 nM and k2 of 0.2 min−1.