Expression of osteoblast lineage-associated mRNAs in vitro. Total RNA was isolated from ASCs (n = 12) and BMSCs (n =11) treated under pre-adipocyte (black) and adipocyte conditions (white) grown in the respective growth media. RT-PCR analysis was performed to detect expression of adipose markers, osteonectin (A), E4BP4 (B), GILZ1 (C), GILZ2 (D), RANKL (E), and osteocalcin (F). The values represent the mean ± standard error bars.