TABLE 1.

Semi-quantitative real-time RT-PCR: total RNA from induced (adipogenesis or osteogenesis) or non-induced human ASC (n=12) and human bone marrow (n = 12) stem cells were analyzed by RT-PCR using the primer sets outlined below, an approach that has been validated in multiple prior studies (Wu et al., 2007, 2008)

Gene	Accession #	Forward	Reverse
Cyclophilin B	M60857	GGAGATGGCACAGGAGGAAA	CGTAGTGCTTCAGTTTGAAGTTCTCA
Adiponectin	NM_004797	GGCCGTGATGGCAGAGAT	TTTCACCGATGTCTCCCTTAGG
aP2	NM_001442	AAAGAAGTAGGAGTGGGCTTTGC	CCCCATTCACACTGATGATCAT
C/EBPα	NM_004364.2	GGGTCTGAGACTCCCTTTCCTT	CTCATTGGTCCCCCAGGAT
Leptin	NM_000230.1	GGTTGCAAGGCCCAAGAA	ACATAGAAAAGATAGGGCCAAAGC
LPL	NM_000237.1	CAGATGCCCTACAAAGTCTTCCA	TGATTGGTATGGGTTTCACTCTCA
PPARγ2	NM_015869	AGGCGAGGGCGATCTTG	CCCATCATTAAGGAATTCATGTCATA
E4BP4	NM_005384	ACATGTTGTCTGTTTGGTGTCTTTTT	ATACA GCCTTCGCATGGACTATC
GILZ1	NM_198057.2	CTGCAACCGCAACATCGA	CGGAGGCACTGTGGAAGAAG
GILZ2	NM_004089.3	GCCATGGATCTGGTGAAGAATC	TCGGATCTGCTCCTTCAGGAT
Osteocalcin	NM_199173.2	GCCCAGCGGTGCAGAGT	TAGCGCCTGGGTCTCTTCAC
Osteonectin	NM_003118	GCGGGACTGGCTCAAGAAC	GATCTTCTTCACCCGCAGCTT
RANKL	NM_003701	CATCCCATCTGGTTCCCATAA	GCCCAACCCCGATCATG

Results for each mRNA were normalized relative to the expression of cyclophilin B as a control.