Table 1. Number of proteins identified from 0.5ug of trypsin digest of protein.

Sample Type	Sample and extraction condition		Identified protein (Mascot)	Identified Protein (SEQUEST)
				Number	Identified Spectra	Total Spectra	%Identified Spectra
unfixed	1	Room Temperature	706	312	8465	19497	43
	2	Room Temperature	629	234	7597	18482	41
	3	Room Temperature	679	302	7948	18720	42
FF (3 months)	1	Room Temperature	112	36	533	6843	8
		Heat only	469	167	5257	18488	28
		Heat + Pressure	585	202	6666	18905	35
	2	Room Temperature	180	74	1230	9616	13
		Heat only	561	217	6273	21752	29
		Heat + Pressure	581	259	7416	21732	34
	3	Room Temperature	106	65	1440	7777	19
		Heat only	359	162	2693	14008	19
		Heat +pressure	527	213	3688	16919	22
FF (15 yrs)	4	Heat only	0	0	0	0	0
		Heart + Pressure	22	12	73	6242	1.2
	5	Heat only	0	0	0	0	0
		Heart+ Pressure	45	21	118	6775	1.8
	6	Heat only	0	0	0	0	0
		Heart+ Pressure	79	28	28	6242	2.0
FFPE (15 yrs)	1	Heat only	471	149	2752	21137	13
		Heat+ Pressure	565	206	6030	22662	27
	2	Heat only	521	198	5456	22602	24
		Heat + Pressure	589	219	7479	22504	33