Table 2. Comparison of peptide and sequence coverage of fibronectin and filament Ain FFPE samples on different extraction condition.
Protein | FFPE Sample and extraction condition | Unique peptide | Unique spectra | Total Spectra | %Sequence coverage | |
---|---|---|---|---|---|---|
Fibronectin | 1 | Heat only | 37 | 39 | 90 | 18 |
Heat+Pressure | 51 | 58 | 215 | 25 | ||
2 | Heat only | 44 | 50 | 244 | 25 | |
Heat + Pressure | 55 | 65 | 345 | 32 | ||
Filament A | 1 | Heat only | 38 | 38 | 87 | 19 |
Heat+ Pressure | 63 | 69 | 299 | 32 | ||
2 | Heat only | 60 | 71 | 355 | 33 | |
Heat + Pressure | 65 | 79 | 419 | 35 |