TABLE 2.
List of phosphoproteins identified using MALDI-TOF mass spectrometer
| Spot | Protein Identity | Accession No.* | Mass | Score | Protein function |
|---|---|---|---|---|---|
| 1 | Rho GDP dissociation inhibitor 1 | gi76780069 | 23250 | 68 | Regulates the GDP/GTP exchange reaction of Rho proteins, regulates actin cytoskeleton organisation. |
| 2–5 | Heat shock protein 27 | gi662841 | 22427 | 105–107 | Involved in stress resistance, regulates actin organization, supports the formation of actin stress fibres, stabilizes the integrity of the cell. |
| 7 | Myosin regulatory light chain 2 | gi15809016 | 19696 | 80 | Regulates cell contraction, but also organization of the actin cytoskeleton. |
| 9 | Calponin 3 | gi19263471 | 36391 | 680 | Part of the actin cytoskeleton, less involved in the contraction process. |
| 11 | Alkaline phosphatase precursor | gi2507183 | 57406 | 65 | Enzyme used for the dephosphorylation experiment. |
*
Mascot search results from the NCBInr protein database.