Figure 3.

Enzymatic One-Pot Conversion of Gentamicin A2 to Gentamicin A

LC-ESI-HRMS selective ion monitoring was carried out on (A) [M + H]⁺ (m/z 456) and [M + Na]⁺ (m/z 478) ions of gentamicin A2 (3); (B) [M + H]⁺ (m/z 469) and [M + Na]⁺ (m/z 491) ions of gentamicin A (6), produced by the coupled action of GenD2, GenS2, and GenN on gentamicin A2 (3). MS/MS fragments from the [M + H]⁺ (m/z 456) and [M + H]⁺ (m/z 469) ions are shown as insets.