Figure 2.

Fluorescence images of mouse seminiferous tubules showing the effects of different pretreatments on the simultaneous use of IHC for 5-bromo-2’-deoxyuridine (BrdU;red) and lectin histochemistry (LHC) for peanut agglutinin (PNA; green). Nuclei were counterstained with Hoechst 33258 (blue). Paraffin sections of the testis were pretreated with HCl (A-C) or heat-induced antigen retrieval (HIAR; D–I). They were then immunostained with the mouse monoclonal anti-BrdU antibody (A–F) or normal mouse IgG (G–I) and, at the same time, were stained with PNA-LHC. Pictures in different combinations of wavelengths are shown. Scale, 25 μm.