Figure 9.

Fluorescence images of mouse seminiferous tubules showing double immunostaining of BrdU (red) and CADM1 (green). Nuclei were counterstained with Hoechst 33258 (blue). Paraffin sections of the mouse testis were pretreated with heat-induced antigen retrieval (HIAR) and then immunostained with the mouse monoclonal anti-BrdU antibody together with the rabbit polyclonal anti-CADM1 antibody. Pictures in different combinations of wavelengths are shown. (A–C) At stage IV, BrdU was localized in intermediate spermatogonia (In) that were simultaneously positive for CADM1 (red arrowheads in A, green arrowheads in B, and yellow arrowheads in C). (D–F) At stage VIII, BrdU was localized in preleptotene spermatocytes (Pl) that were simultaneously positive for CADM1 (red arrowheads in D, green arrowheads in E, and yellow arrowheads in F). (G–I) At stage XII, BrdU was localized in a differentiated A3 spermatogonium (A3) that was negative for CADM1 (red arrowhead in G and I). Scale, 25 μm.