FIG. 2.
Preincubation of skin explants with RANTES analogues blocks subsequent R5 HIV infection in LC in a dose-dependent manner. LC within skin explants were preincubated with the indicated concentrations of RANTES analogues for 20 min, exposed to HIVBa-L for 2 h, cultured for 3 days, and then double stained with anti-p24 and anti-major histocompatibility complex class II MAbs. (A) Representative fluorescence-activated cell sorter analyses from a single experiment using AOP-RANTES. (B) Summary of nine experiments using skin explants from nine individuals with various CCR5 diplotypes. Each line represents data from a different individual skin donor.