FIG. 4.
PSC-RANTES is more potent than AOP-RANTES and NNY-RANTES in its ability to block R5 HIV infection of LC. LC within skin explants were preincubated with a suboptimal blocking dose (10 nM) of each RANTES analogue for 20 min, exposed to HIVBa-L for 2 h, cultured for 3 days, and then stained with anti-p24 and anti-major histocompatibility complex class II MAbs. (A) Representative fluorescence-activated cell sorter analyses from a single experiment showing comparative blocking ability of each analogue. (B) Summary of 27 separate comparative experiments using LC from 27 skin donors. Percent inhibition of LC infection was calculated as described in Materials and Methods.