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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Immunol. 2015 Feb 2;194(5):2369–2379. doi: 10.4049/jimmunol.1402412

Figure 3. MDSCs from IRF8 MKO mice exhibit decrease spontaneous apoptosis.

Figure 3

A. MDSCs from IRF8 KO mice exhibit decreased spontaneous apoptosis. Spleen and bone marrow were collected from three month-old WT (n=3) and IRF8 KO (n=3) mice. Cells were stained immediately under cold conditions with CD11b- and Gr1-specific mAbs plus Annexin V and DAPI. CD11b+Gr1+ cells were gated for Annexin V+DAPI+ cells. Shown are representative results of one of 3 pairs of mice. Apoptosis was quantified as % CD11b+Gr1+ cells that are also Annexin V+DAPI+ cells and presented at the right. Column: mean; bar: SD. ** p<0.01. B. MDSCs from IRF8 MKO mice also exhibit decreased spontaneous apoptosis. Spleen and BM cells were collected from WT (n=5) and IRF8 MKO (n=5) mice. Spontaneous apoptosis were determined as in A.