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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Immunol. 2015 Feb 2;194(5):2369–2379. doi: 10.4049/jimmunol.1402412

Figure 9. GM-CSF is not down-regulated in MDSCs from IRF8 KO mice.

Figure 9

CD11b+Gr1+ MDSCs were purified from spleens of WT (n=4) and IRF8 KO (n=3) mice. Total RNA was isolated from these purified MDSCs and analyzed for IRF8 expression levels by real-time RT-PCR using β-actin as the internal control. The IRF8 expression level in MDSCs from the first WT mice was arbitrarily set at 1.0. The expression levels of IRF8 in MDSCs of the rest samples were the levels relative to the IRF8 expression level in MDSCs of first WT mice.