Table 1. DPPH and nitrite radical scavenging activities and reducing power of water and methanol extracts from N. nucifera leaves.
Sample (μg ml−1) | *Control (μg ml−1) | DPPH radical scavenging activity (%) | Nitrite radical scavenging activity (%) | Reducing power activity (absorbance at 700 nm) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
Water extract | Methanol extract | Ascorbic acid | Water extract | Methanol extract | Ascorbic acid | Water extract | Methanol extract | Ascorbic acid | ||
100 | 50 | 0.59±0.42 | 16.84±1.54 | 22.07±0.66 | 5.41±1.47 | 14.19±0.49 | 15.6±2.76 | 0.08±0.00 | 0.12±0.00 | 0.27±0.01 |
200 | 100 | 5.19±0.88 | 25.13±0.29 | 47.58±0.42 | 12.75±0.62 | 21.33±1.94 | 59.29±2.34 | 0.11±0.00 | 0.22±0.00 | 0.51±0.02 |
500 | 150 | 16.29±1.70 | 56.26±2.23 | 71.64±0.21 | 23.20±1.10 | 47.43±2.07 | 88.94±2.30 | 0.16±0.01 | 0.76±0.01 | 0.80±0.01 |
1000 | 200 | 28.30±1.13 | 86.67±0.20 | 93.00±0.24 | 43.42±1.14 | 54.76±1.84 | 97.05±0.54 | 0.20±0.02 | 0.89±0.01 | 1.06±0.02 |
** IC50 (μg/ml) | 1699.47 | 514.36 | 106.48 | 1141.33 | 784.02 | 95.51 | - | - | - |
All data are expressed as the mean ± SD (n = 3).
*Ascorbic acid was used as a positive control for the DPPH radical scavenging, nitrite radical scavenging, and reducing power activity assays.
** IC50 (μg ml−1): concentration at which 50% of the activity is inhibited.