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HSV glycoprotein requirements for infection of cells that support distinct entry pathways. HeLa, CHO-nectin-1, or Vero cells were infected with HSV-1 strain KOS mutants devoid of gB, gC, gD, or gL for 10 h. Total cell number and HSV antigen-positive cells were quantitated by immunofluorescence microscopy. Four-hundred to 500 cells per sample were evaluated. One-hundred percent represents the infectivity of an equivalent number of particles of the corresponding complemented virus.
