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. 2015 Feb 21;15:40. doi: 10.1186/s12866-015-0371-2

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© Kang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of tyrosine phosphorylated GST-Wze released in the wash fractions. GST-Wze in wash fractions in the presence of 10 mM ATP (1–3 and 7–9) and absence of ATP (4–6 and 10–12) from interaction of GST-Wze with resin-bound His₆-Wzd expressed by E. coli BL21(DE3): (A) Coomassie stained 12% SDS-PAGE gel and (B) Western blot probed with mouse monoclonal anti-phosphotyrosine antibody. GST-Wze in wash fractions in the presence of 10 mM ATP (13–15 and 19–21) and absence of ATP (16–18 and 22–24) from interaction of GST-Wze with His₆-Wzd expressed by E. coli C41: (C) Coomassie stained 12% SDS-PAGE gel and (D) Western blot probed with mouse monoclonal anti-phosphotyrosine antibody.