Table 2.
Primer 1 | Sequence (5’ to 3’) | Gene target |
---|---|---|
WzdRSacI | CGg agc tcA AGA GCA AAT TGA CCT TGC AC | wzd |
WzdFXbaI | CGt cta gaT ACT TAA ACG CGT CTC CGG CTT CG | wzd |
WzeRXbaI | GCG tct aga TTG AGG AGA AAA AAC ATG AAT TTT TC | wze |
WzeF-Wzb | ATA CTA TCT AAG CTC AAT ACT TAA ACG CGT CTC CGG | wze |
WzbR-Wze | ATA AAT AGC ATG CCT TAG ATA GTA TTG GAA GGG GAA C | wzb |
WzbFAatII | CGg acg tcA TGA AAT TAG CAC TCG CAC AAC C | wzb |
WzdSBamI | CGg gat ccA TTG ACC TTG CAC GAC TTT GG | wzd |
WzdRXhoI | CCG ctc gag TAC TTA AAC GCG TCT CCG GC | wzd |
WzeSBamI | CGg gat ccT CAT TAG AGA AAA TTT TGC ATA GAC | wze |
WzeRXhoI | CCG ctc gag GAC AGT TAG AAG CGC ATG CT | wze |
WzbSEcoI | Gga att cGA TTG ATG TGC ATT GCC ATA TG | wzb |
WzbRXhoI | CCG ctc gag TAC CTT AAT ACC GCG ACA ACA AAC | wzb |
WzeRhamS | CGg gat cca GAC GCG TTT AAG TAT TGA GGA G | wze |
WzeRhamR | CCC aag ctt TGA CAG TTA GAA GCG CAT GC | wze |
1Restriction sites in primers are indicated in lower case letters.