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. 2015 Feb 19;12:15. doi: 10.1186/s12950-015-0060-9

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© de Gaetano et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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CLA primes monocytes to an anti-inflammatory MΦ2 macrophage phenotype. RT-PCR analysis of (a) CD14 and CD68 and (b) CD163 and MR mRNA expression in or M-CSF stimulated (100 ng/ml) differentiating HPBMCs following treatment with c-9,t-11-CLA, t-10,c-12-CLA, CLA blend (80:20 c-9,t-11:t-10,c-12), OA, LA or TROG. In unstimulated conditions, both CLA isomers and their blend decrease CD14 expression and increase expression of both CD163 and MR, without affecting CD68 expression. Following M-CSF stimulation, c-9,t-11-CLA and CLA blend decrease the mature macrophage marker CD68 and increase expression of both CD163 and MR (MΦ2 markers). Data are mean +/− SEM of three independent experiments. Data is expressed as fold change in gene expression relative to DMSO control, where *p < 0.05; ** p < 0.01 and ***p < 0.001 vs DMSO.