FIG. 2.

High-fat dietary restriction (HFDR) induces Lamp2a, a lysosomal protein involved in chaperone-mediated autophagy (CMA). Hepatic protein levels of the CMA Lamp2a (A) and the macro-autophagy markers LC3-I and II (B) as well as the unfolded protein response marker BiP/Grp78 (C) were determined by western blotting, and densitometric analysis of target bands of DR was compared to ad libitum–fed (AL) mice. Target protein expression was related to the total protein fluorescence assessed on the blotting membrane. Representative blots from two out of six animals per groups are shown. Densitometric values are means+standard error of the mean (SEM) from six animals per group. Proteasome activity was measured in liver lysates of all animals and group means+SEM are shown (D). (*) Significant difference (p<0.05 by t-test) between AL and DR mice.