Induction of ISG expression during WNV-NY infection. (A) Northern blot analysis of ISG6-16, ISG20, ISG15, ISG56, and GAPDH expression in cells that were mock infected or infected with WNV-NY for the indicated hours (hr). (B) Steady-state protein levels of IRF-9, STAT1α, ISG15, ISG56, and actin in mock- or WNV-infected 293 cells were examined by immunoblot analysis of extracts prepared at the indicated times postinfection.