FIG. 2.

Quantitative analysis of yeast three-hybrid interactions. Yeast cells were transformed with the MMLV Gag prey expression vector, along with various RNA bait expression vectors indicated on the x axis. Regions of MMLV present in RNA baits are indicated by nucleotide positions relative to the retroviral genome. Reporter activation was quantified by β-galactosidase assay and given in Miller units (see Materials and Methods). Values are means ± standard deviations (error bars) of at least three independent cultures.