TABLE 2.
Mrp families of GAS defined by antibody reactivity with whole bacteria
| Mrp antibody bindinga | GAS Emm type |
|---|---|
| Anti-MrpI | 2, 65NA, 69, 92, 102, 114, 117, 118 |
| Anti-MrpII | 4, 8, 25, 28, 30, 32, 33, 36, 41, 43, 53, 58, 60, 64, 65Mali, 66, 67, 70, 71, 72, 74, 78, 79, 80, 82, 83, 86, 88, 89, 91, 93, 95, 97, 98, 100, 101, 105, 108, 109, 111, 115, 116, 120, 121, 124 |
| Anti-MrpIII | 9, 11, 22b, 44/61, 49, 52, 59, 68, 73, 84, 85, 94, 96, 106, 107, 112, 113 |
| Anti-Mrp full length but not anti-Mrp N-terminal region | 48, 75, 87, 99, 104, 110 |
| No antibody bindingc | 12, 13, 31, 34, 42, 50, 56, 76, 77, 81, 122 |
a
Binding of antibodies was considered positive if ELISA values were >0.1 after subtracting the background OD observed with preimmune rabbit serum. Average ODs of antisera were as follows: Mrp2N antiserum, 0.87 (range, 0.22 to 1.78); Mrp4N antiserum, 0.86 (range, 0.19 to 1.52); Mrp49N antiserum, 0.49 (range, 0.12 to 0.85).
b
Laboratory strain of type emm22 reacted with anti-MrpIII antiserum, but the GenBank sequence aligned with MrpII.
c
emm types predicted to be Mrp negative that were tested and resulted in no antibody binding were M3, M6, M14, M18, M19, and M24.