FIG 2.
Immunization with VacSIM increases rHBsAg-specific antibody titers in both Th1- and Th2-biased mouse models. Comparison of rHBsAg-specific antibodies induced in mice immunized 3 weeks apart. The immunized and naïve groups had 5 and 4 mice per group, respectively. There are only 3 IgM values for rHBsAg + CpG-immunized C57BL/6 mice, as sera were limited. Serum was collected 3 weeks postboost, and rHBsAg-specific IgM, IgG, IgG1 and IgG2b endpoint titers were determined by indirect ELISA. Dashed lines indicate detection limits of the assay. Statistical differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001) were determined by one-way ANOVA with the Bonferroni posttest.