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. 2015 Feb 25;22(3):336–343. doi: 10.1128/CVI.00714-14

FIG 3.

FIG 3

Cell-mediated immunity is induced following immunization via VacSIM. Mice were immunized 4 weeks apart and rHBsAg-specific CD8+ T cells were enumerated by IFN-γ ELISpot (A) and flow cytometry (B) of splenocytes harvested from mice (n = 5, except n = 1 for rHBsAg + Freund's, due to lost samples) at 3 weeks postprime (left) and 2 weeks postboost (right).