Fig 4. Developmental and tissue distribution of BmSODs in B.mori .
Aliquots (10 μg) of whole body homogenates from B. mori of the following stages, separated by SDS-PAGE, transferred to nitrocellulose and probed with anti-SOD antibodies (A): day 0 of the first (lane 1), second (lane 2), third (lane 3), fourth (lane 4) and fifth (lane 5) instar larvae, as well as pupae (lane 6) and adult (lane 7) stages. Aliquots (10 μg) of protein from various tissues of day 3 fifth instar larvae were subjected to SDS-PAGE and were examined for expression of both BmSODs antibodies (B): midgut (lane 8), silk gland (lane 9), testis (lane 10), fat body (lane 11), ovary (lane 12) and Malpighian tubule (lane 13).