TABLE 1.

Primer used to amplify overlapping short fragments tth3, tth4, tth5, tth6, and tth7

Fragment	Primer	Primer sequence (5′-3′)	Annealing temp (°C) and time (s)	Product size (bp)
tth3	af1h3	TGG GCA CCA AAA CCC CAA GAG AAA GTC AG	70, 30	606
	air1h3	TTG TGA CCC CTC CCC AGT CAG GTG ACT TGT G
tth4	af1h4	AAA CAG AGT GTA CCT TTT CCC CCC CGA C	70, 30	776
	ar1h4	TTG ACT TCC GGG TTA TAC GGC AAC CCT C
tth5	af1h5	ATT GTT AGA GTA GGC CCC GAG CAG TG	65, 90
	ar1h5	TTT TGA GTA GGT GTG GCT GAT GGT G
	if1h5 (nested)	CGA GGA ATA CAC TGC CTG TAA ATA CTG		594
	ir1h5 (nested)	GTG GCT GAT GGT GAC CTT TGA ACT C
tth6	af1h6	TAT CTG TTC CCA GAA CGC TTA CCA C	60, 30
	arir1h6	GCT TAC TTA AAA TGG CGG CCA TGA C
	if1h6 (seminested)	ACA TAC CCA GAA ACC CAG GCT TCA G	65, 30	495
tth7	af1h7	ATG CCT ACA GTA GGT CCC AGG CAG TG	60, 30
	ar1h7	GTG GCT GAT GGT GAC CTT TGA ACT C
	if1h7 (nested)	AGA AAG TCA GCC CCA GCT CCT AGC AG		620
	ir1h7 (nested)	TGA CCT TTG AAC TCA CGC CAC CGT C