Table 2. Primer sequences used for quantitative real-time polymerase chain reaction.
| Gene | Amplicon 1 | Temperature (ºC) 2 | Primers sequence (5’- 3’) |
|---|---|---|---|
| GHR 3 | 145 | 60°C | AACACAGATACCCAACAGCC |
| AGAAGTCAGTGTTTGTCAGGG | |||
| IGF-I | 140 | 60°C | CACCTAAATCTGCACGCT |
| CTTGTGGATGGCATGATCT | |||
| PI3KR1 | 145 | 60°C | GCCCTCTCCTTTTCAAAT |
| ACAGTATTAGGTTTCGGTGGC | |||
| CTSL2 | 80 | 60°C | GAAGTCAGAAAGGAAGTACAGAGG |
| CTCTCCAGTCAACAGATCGTG | |||
| Atrogin-1 | 174 | 60°C | CCAACAACCCAGAGACCTGT |
| GGAGCTTCACACGAACATGA | |||
| β- actin | 136 | 60°C | ACCCCAAAGCCAACAGA |
| CCAGAGTCCATCACAATACC |
1Amplicon (bp)
2Annealing Temperature (°C)
3CTSL2, cathepsin L2; GHR, growth hormone receptor; IGF-I, insulin-like growth factor I; PI3KR1, phosphatidylinositol 3-kinase, regulatory 1.