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. 2015 Feb 25;10(2):e0118322. doi: 10.1371/journal.pone.0118322

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© 2015 Eckdahl et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Junction-Golden Gate Assembly (J-GGA) introduces genetic variation into a single gene expression cassette as shown, or multiple gene expression cassettes arranged in tandem. PCR amplifies the vector and adds BsaI restriction sites and sticky ends complementary to the elements to be inserted. J-GGA inserts element(s) using standardized PCR primers regardless of the insert sequences. (B) The online Golden Gate Assembly Junction Evaluative Tool (GGAJET) enables users to design junctions with compatible sticky ends and specific primers with similar melting temperatures. GGAJET is available at gcat.davidson.edu/SynBio13/GGAJET/.