FIG. 4.
Effects of inhibitors on PLA2 activity of AD169 HCMV. HCMV (AD169, 106 PFU) was incubated with MAFP, HELSS, pyrrolidine 1, and indoxam. Virus was then washed twice by ultracentrifugation after treatment and incubated with BODIPY-PC and NBD-PC. Error bars represent standard deviations of the mean from three independent experiments. Lipid extraction and HPLC analysis of fluorescent metabolites were performed as described in Materials and Methods. One hundred percent relative hydrolysis was used to represent the hydrolysis of fluorescent substrates observed in the presence of HCMV. The absolute values ranged from 3 to 7% for NBD-PC and 4 to 12% for BODIPY-LPC.