FIG. 6.

Western blot analyses of cPLA2 in MRC5 cells and HCMV. Samples (cells, 50 μg; and AD169, 100 μg) were analyzed by Western blotting with a cPLA2α-specific MAb (A). The presence of cPLA2α in purified virus (Towne strain; protein amount, 300 μg) was tested, using a cPLA2α-specific MAb, in comparison with various amounts of total proteins from MRC5 cells (125, 100, 75, and 50 μg) (B). Proteins (150 μg/sample) from cells and HCMV (AD169) were separated in two phases with Triton X-144. Thirty micrograms of proteins from supernatant (containing cytosolic proteins) and pellet (containing membrane proteins), respectively, was analyzed in a Western blot with cPLA2α-specific MAb (C).