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CLSM on MHV-SGFP and MHV-WT. Virus obtained from ∼107 infected LR7 cells was pelleted through a 20% sucrose cushion and resuspended in 100 μl of PBS. A 10-μl aliquot of either MHV-WT (A) or MHV-SGFP (B) was examined by CLSM (×100 magnification).