Fig. 2.

VHH specificity for N-terminal htt. Assays were performed on a recombinant N-terminal htt fragment consisting of amino acids 15–378 with a polyQ length of 17 (htt a.a. 15–378 Q17). Positive control: MAB5492. Negative control: No P-VHH or P-nVHH. a ELISA on wells with (black bars), or without (white bars) N-terminal htt. Bars represent mean ELISA signal from two independent ELISA assays with standard deviation. Each assay was performed in triplicate. ELISA absorption units are measured at λ = 490 nm. b Western blotting on N-terminal htt. Blots were performed twice. kDa Molecular weight (kilodalton)