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Construction of N-terminal Htt fragments
| N-terminal htt fragment | Forward primer | Reverse primer |
|---|---|---|
| a.a. 1–318 (Q17/Q43) | TATGGCGACCCTGGAAA | GTCGACGAGCAGCACGCCAAGA |
| a.a. 15–378 (Q17/Q43) | CAAGTCCTTCCAGCAGCA | GTCGACGGCTCCGGTCACAACA |
| a.a. 49–415 (no polyQ) | GCCGCCTCCTCAGCTTC | GTCGACGCCACCAGACTCCTCCTT |
a.a. amino acid, Q17/Q43 polyQ stretch, Underscored SalI-site
