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Experimental setting. The RIPC-protocol consisted of 4 × 5 min inflation/deflation of a blood pressure cuff. Plasma was obtained before (T0), directly after (T1) and 60 min after (T2) the RIPC stimulus. HUVEC cells were incubated with the respective plasma and subjected to 24 h of hypoxia. Employing cell culture media and cell lysates, cell damage as well as cellular signalling events were investigated. H hypoxia; green color, RIPC-plasma
