FIG. 4.

Detection of the K14 protein on HHV-8-harboring cells undergoing lytic induction. (A) Flow cytometry of BC-3 cells undergoing the lytic cycle and stained with MAb OX112 (bold line) compared to an isotype-matched control MAb, OX1 (shaded histogram). (B) CD200R-coated fluorescent beads bind BC-3 cells (thick line) in increasing amounts following induction of lytic replication of HHV-8 compared to a negative control (CD4d3 + 4-coated beads) (shaded histogram). The cells became larger and more granular, consistent with lytic induction. The cells shown in the labeling were gated on the viable population. Results from one typical experiment of five are shown.