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. 2015 Feb 25;6:49. doi: 10.3389/fimmu.2015.00049

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Copyright © 2015 Sharma, Khosla, David, Rastogi, Vyas, Singh, Bhardwaj, Sahney, Maiwall, Sarin and Trehanpati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Representative flow-cytometric dot plots showing the expression of IL-10 and TGF-β in Tregs in three patient groups. Scatter plot shows that both IL-10 and TGF-β were expressed significantly more in Tregs of HBV-HCC patients than both non-HBV-HCC and CHBV. (C) qRT-PCR analysis confirmed the overexpression of TGF-β in HBV-HCC than non-HBV-HCC. (D) Immunohistochemistry analysis also confirmed the over expression of TGF-β in HBV-HCC than non-HBV-HCC liver tissues. 40× magnification was used.