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. 2004 Jul;78(14):7344–7351. doi: 10.1128/JVI.78.14.7344-7351.2004

FIG. 4.

FIG. 4.

Functional budding assay for coexpressed VP40 and NP. 293T cells were transfected with pCAGGS vector alone (lane 1), NP (lane 2), VP40 (lane 3), or VP40 plus NP (lane 4). Radiolabeled cell extracts (A) and VLPs (B) were immunoprecipitated with appropriate antiserum, and immunoprecipitated proteins were analyzed by SDS-PAGE and autoradiography.