FIG. 1.

CEACAM1a expression on microglia from adult mice. Single-cell brain suspensions from naïve mice were stained with anti-CD45, anti-CEACAM1a, or isotype control MAb. In the top row are representative density plots showing forward-side scatter and staining patterns. R1 indicates the live cell gate used for analysis. CD45 expression differentiates CD45^low microglia and CD45^high infiltrating cells (combined in R2). R3 and R4 highlight CD45^low microglia stained with CC-1 or isotype control MAb. Shown in the bottom row is CEACAM1a expression (light lines) on microglia from C57Bl/6 and BALB/c mice but not SJL mice (R3). Dark lines represent isotype control MAb (R4); numbers are MFI values for CEACAM1a (right) and the isotype control (left).