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Replicon constructs. (A) Sites and amino acid sequences of the GFP insertions in NS5A. (B) Replicon constructs. Cell culture adaptive mutations in NS3 and NS4B (GIT constructs) or NS5A (S2204I constructs) are marked by circles. The pol− control constructs harbor an inactivating GDD→AAG mutation in the NS5B RNA-dependent RNA polymerase.
