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NS5A-GFP can be directly visualized by fluorescence microscopy. Huh-7.5 cells harboring the constructs GIT/5A-GFP-6 (A) and I/5A-GFP-6 (B) were analyzed by fluorescence microscopy at 5 days postseeding. (C) Cells harboring the parental S2204I replicon without GFP insertion served as the negative control.
