FIG. 5.

Relationship between symptom severity, recovery, and siRNA accumulation in ICMV-infected N. benthamiana and in EACMV-[UG]-infected cassava plants. (A) ICMV symptom severity and siRNA accumulation trend in N. benthamiana. (B) RNA gel blot probed with [∝-³²P]dCTP-labeled DNA-A and DNA-B of ICMV in N. benthamiana at days 2 and 4 in inoculated leaves and at weeks 1 to 8 in systemically infected leaves. (C) Representative leaves showing different degree of symptoms and recovery phenotype at 2, 4, 6, and 8 wpi in N. benthamiana. (D) EACMV-[UG] symptom severity and siRNA accumulation curves in cassava plants. (E) RNA gel blot probed with [∝-³²P]dCTP-labeled DNA-A and DNA-B of EACMV-[UG] in cassava plants at days 2 and 4 in inoculated leaves and at weeks 1 to 8 in systemically infected leaves. (F) Representative leaves showing different degree of symptoms and recovery phenotype at 2, 4, 6, and 8 wpi in cassava plants. A total of 15 N. benthamiana plants for ICMV and 15 cassava plants for EACMV-[UG] were inoculated as five plants in three experiments. Ethidium bromide-stained RNA is shown as a loading control. Bars in symptom severity curve indicate the SE values of 15 plants. The C within the blots and leaf panels represents a mock-inoculated, control plant.