FIG. 7.

Transcript overlap at the 3′ ends of CP and AC3 genes in ACMV-[CM]. (A) Schematic representation of virion-sense CP gene and complementary-sense AC3 gene overlapped by 4 nt in ACMV-[CM]; (B) Northern blot analysis of total RNA (5 μg) isolated from ACMV-[CM]-infected N. benthamiana. An RNA blot (left) hybridized with CP strand-specific [γ-³²P]ATP-labeled 70-mer (oligonucleotide cAV2), and the RNA blot (right) hybridized with AC3 strand-specific [γ-³²P]ATP-labeled 70-mer (oligonucleotide cAC3) as the probe. Ethidium bromide-stained rRNA at the bottom of the blot serve as a loading control.